Browsing by Author "Chawinga, Kenneth"

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    Comparative analysis of antimicrobial resistance profil E.coli isolated from intensively reared and free range chickens in selected districts of Zambia
    (Kamuzu University of Health Sciences, 2020-11-11) Chawinga, Kenneth
    Type of study: A cross-sectional study Aim of the study: To investigate and compare the antimicrobial resistance profile of E. coli isolated from intensively reared and free range chickens Specific objectives: 1. To isolate ESBL producing E. coliisolates from commercial broiler and free-range retail chickens 1.To determine the carriage of ESBL-Producing commensal E. coliin healthy intensively and free range chikens 2. To determine antimicrobial sensitivity of the isolates to commonly used antimicrobials 1. To determine presence of antimicrobial resistant genes in the isolated strains.2.To determine the phenotypic and genotypic characterization of antimicrobial resistance in isolated strains. 3.2. 3. To asses the diversity of antimicrobial usage at farm/household level. Problem statement: Extended-spectrum beta-lactamases were found first in Klebsiella pneumonia and then predominantly in E. coli, making the later an important group of bacteria in dissemination of antibiotic resistant genes, mainly from the AMR Profile of E.Coli in Chickens COMREC Application Version 2.0_02/11/2020 COMREC Application diverse compounds and widely used therapeautic class of antibacterials due to their excellent safety profile and broad antimicrobial spectrum. This, unfortunately, has also given rise to a continuous increased resistance,globally in health care settings as well as in the community, due to their permanent ability to cause antimicrobial resistant genes to become more common in the population (Ur Rahman et al. 2018). Extended-spectrum beta-lactamase (ESBL) producers are Gram-negative bacteria that produce enzymes that impart resistance to most beta-lactam antibiotics such as penicillins, cephalosporins, and the monobactam aztreonam (Chishimba et al. 2016). This cross sectional study ,of comparative analysis of antimicrobial resistance profile of E. colithat will be isolated fromapparent healthyintensively reared and free range chickens in Kabwe and Kapiri Mposhi Districts located in Central Province in Zambia, shall endeavor to bring out evidence of; 1. Presence of ESBL producing E. Coli in the gastrointestinal tract of free range and intensively kept chickens1.To determine the carriage of ESBL-Producing commensal E. coliin healthy broilers and freerange 2. Antimicrobial sensitivity of the isolates to commonly used antimicrobials and 3. Presence of antimicrobial resistant genes in the isolated strains 2.To determine the phenotypic and genotypic characterization of antimicrobial resistance in isolated strains. 3. To asses the diversity of antimicrobial usage at farm/household level.