Comparative analysis of antimicrobial resistance profil E.coli isolated from intensively reared and free range chickens in selected districts of Zambia
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Date
2020-11-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Kamuzu University of Health Sciences
Abstract
Type of study:
A cross-sectional study
Aim of the study:
To investigate and compare the antimicrobial resistance profile of E. coli
isolated from intensively reared and free range chickens
Specific objectives:
1. To isolate ESBL producing E. coliisolates from commercial broiler and
free-range retail chickens 1.To determine the carriage of ESBL-Producing
commensal E. coliin healthy intensively and free range chikens
2. To determine antimicrobial sensitivity of the isolates to commonly used
antimicrobials
1. To determine presence of antimicrobial resistant genes in the isolated
strains.2.To determine the phenotypic and genotypic characterization of
antimicrobial resistance in isolated strains.
3.2. 3. To asses the diversity of antimicrobial usage at farm/household
level.
Problem statement:
Extended-spectrum beta-lactamases were found first in Klebsiella pneumonia and
then predominantly in E. coli, making the later an important group of bacteria in
dissemination of antibiotic resistant genes, mainly from the
AMR Profile of E.Coli in Chickens COMREC Application Version 2.0_02/11/2020 COMREC Application
diverse compounds and widely used therapeautic class of antibacterials due to their
excellent safety profile and broad antimicrobial spectrum. This, unfortunately, has
also given rise to a continuous increased resistance,globally in health care settings
as well as in the community, due to their permanent ability to cause antimicrobial
resistant genes to become more common in the population (Ur Rahman et al.
2018). Extended-spectrum beta-lactamase (ESBL) producers are Gram-negative
bacteria that produce enzymes that impart resistance to most beta-lactam
antibiotics such as penicillins, cephalosporins, and the monobactam aztreonam
(Chishimba et al. 2016). This cross sectional study ,of comparative analysis of
antimicrobial resistance profile of E. colithat will be isolated fromapparent
healthyintensively reared and free range chickens in Kabwe and Kapiri Mposhi
Districts located in Central Province in Zambia, shall endeavor to bring out
evidence of;
1. Presence of ESBL producing E. Coli in the gastrointestinal tract of
free range and intensively kept chickens1.To determine the carriage
of ESBL-Producing commensal E. coliin healthy broilers and freerange
2. Antimicrobial sensitivity of the isolates to commonly used
antimicrobials and
3. Presence of antimicrobial resistant genes in the isolated strains
2.To determine the phenotypic and genotypic characterization
of antimicrobial resistance in isolated strains.
3. To asses the diversity of antimicrobial usage at farm/household
level.
Description
Keywords
ANTIMICROBIAL RESISTANCE