The effect of repeated washing of the royal guard, Interceptor G1 and G2 nets on behaviour (Host seeking and blood feeding) and survival of anopheles mosquitoes
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Date
2022-08-17
Authors
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Kamuzu University of Health Sciences
Abstract
Insecticide treated mosquito nets on behaviour and survival of Anopheles mosquitoes the
effect of repeated washing of the royal guard and interceptor G2 nets on behaviour (host
seeking and blood feeding) and mortality on Anopheles mosquitoes. This is a sub study
which will be conducted under ESSENTIALS project IRB number P.10/20/3155 being
hosted by the Malaria Alert Centre in Blantyre.
Problem statement
Insecticide-treated nets remain important in preventing transmissions of mosquito borne
pathogens. Long-lasting insecticide-treated nets (LLINs) offer longer time protection against
such bites because they are more wash resistant, and are preferred to conventionally treated
nets. Whether the new LLINs (IG2 and RG) are wash resistant compared to the conventional
nets is unknown. Moreover, how the repeated washing affects host seeking and feeding
behaviour and survivor of Anophelines success remains elusive. Therefore, this study will
assess the effect of Royal guard, Interceptor G1 and G2 on blood feeding, host seeking
behaviours and survival of the effect of repeated washing of insecticide treated mosquito
nets on behaviour and survival of Anopheles mosquitoes after repeated washing under
laboratory conditions
Main objective
To assess the effect of repeated washing of insecticide treated mosquito nets on behaviour
and survival of Anopheles mosquitoes
To assess the effect of repeated washing of the royal guard, interceptor g1 and g2 nets on
host seeking and blood feeding behaviour and survival of Anopheles mosquitoes
Specific objectives
1. To determine the number of knocked down and dead mosquitoes post exposure to
both washed and unwashed nets
2. To determine the number of blood fed mosquitoes post exposure to washed and un
washed nets.
3. To evaluate the host seeking behavior of mosquitoes post exposure to washed and un
washed net.
1. To assess the knockdowns and survival of mosquitoes when exposed to
unwashed nets and washed nets.
2. To determine the number of blood fed mosquitoes post exposure to washed
and un washed nets.
3. To evaluate the host seeking behaviour of mosquitoes post exposure to
washed and un washed net.
Methodology
The researcher will conduct a cross-section study and will perform host seeking and WHO
Cone bioassays on Kisumu, Anopheles funestus and Anopheles gambiae. Data will be collected and recorded in a laboratory note book. Collected data will be manually entered
into Microsoft excel and analyzed using R software version 20.
Expected Findings and Dissemination
The researcher hypothesizes that blood fed mosquitoes and host seeking behaviour will
increase with increase in number of net washes while mortality of mosquitoes will decrease
with increase in the number of net washes. Findings from this study will be disseminated
through peer-reviewed publications, presentations at scientific conferences including the
KUHeS annual dissemination conference. A researcher will submit a project completion
report to the College of Medicine Research and Ethics Committee and Liverpool school of
tropical medicine.