The prevalence of Plasmodium falciparum histidine rich protein 2 and histidine rich protein 3 genetic deletions and its association with malaria severity among Malawian children aged 1 to 12 years.
| dc.contributor.author | Mvula, Godfrey Zondwayo | |
| dc.date.accessioned | 2021-12-21T11:56:32Z | |
| dc.date.available | 2021-12-21T11:56:32Z | |
| dc.date.issued | 2021-07-15 | |
| dc.description.abstract | Type of study: This is a quantitative cross-sectional study which will use samples from children diagnosed with malaria in the period from December 2018 to June, 2021.The patients will be categorized in three groups based on the malaria severity; asymptomatic cases, uncomplicated cases and severe malaria cases. The Problem The World Health Organization(WHO) recommended the use of Rapid Diagnostic Tests(RDTs) which target Histidine Rich Protein 2 and Histidine Rich Protein 3 gene (HRP 2/3) to detect malaria. Recent reports of the emerging spread of mutant parasites that fail to express Plasmodium HRP2/3 has complicated the usefulness of RDT’s as Plasmodium infections may be missed and contribute to poor management of malaria especially in vulnerable groups like children. Objective To determine the prevalence of Plasmodium falciparum (Pf) HRP2/3 genetic deletions and its association with malaria severity among Malawian children with a malaria. Methodology The proposed study will use cross-sectional study design to determine the prevalence of HRP2/3 genetic deletion and its association with malaria severity among children aged 1 to 12 years diagnosed with malaria at Queen Elizabeth Central hospital and Ntaja Health center. The Polymerase chain reaction(PCR) also known as PfHRP2/3 deletion assay will be used to determine presence or absence of Plasmodium falciparum strains carrying HRP deletion genes. The descriptive statistics will be used to describe the characteristics of children recruited in the study. The prevalence of PfHRP2/3 will also be presented with 95 percent confidence interval. The chisquared test will be performed to see the association of HRP2/3 deletion with each disease severity group. Furthermore, logistic regression analysis will be conducted to determine the association of malaria severity with the HRP2/3 gene deletion. Expected findings and dissemination We anticipate to find PfHRP2/3 deletion genes in Plasmodium falciparum that affects the performance of HRP2/3 based RDTs and its association with severity of malaria disease. The results will be shared with College of Medicine Research Ethics Committee(COMREC), policy makers, Blantyre Malaria Project(BMP) and publish in journal papers. | en_US |
| dc.identifier.uri | http://rscarchive.kuhes.ac.mw/handle/20.500.12988/718 | |
| dc.language.iso | en | en_US |
| dc.publisher | Kamuzu University of Health Sciences | en_US |
| dc.relation.ispartofseries | Protocol;P.06/21/3332 | |
| dc.subject | Research Subject Categories::MEDICINE | en_US |
| dc.title | The prevalence of Plasmodium falciparum histidine rich protein 2 and histidine rich protein 3 genetic deletions and its association with malaria severity among Malawian children aged 1 to 12 years. | en_US |
| dc.type | Plan or blueprint | en_US |